Development of a new protein- and hormone-free medium for hybridoma cultivation.
نویسندگان
چکیده
منابع مشابه
Growth of NS0 cells in protein-free, chemically defined medium.
Many hybridoma and recombinant myeloma cell lines have been successfully adapted to growth in protein-free media. Compared with serum-supplemented media, use of protein-free media promotes superior cell growth and protein expression and facilitates downstream purification of the expressed product. Owing to its sterol auxotrophy, the NS0 myeloma is normally grown in either a serum-supplemented m...
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inhibitor of the lipid peroxidation induced by iron salts and ascorbic acid.7) It has been revealed that mammalian cells in culture do not always Tequire transferrin, ifiTon is provided as a complex with appropriate chelators.8) To design a protein-free medium for hybridomas, we studied the effect of ferric citratc as an alternative to transferrin on thc proliferation of hybridomas. Mouse hybri...
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عنوان ژورنال:
- Agricultural and biological chemistry
دوره 55 6 شماره
صفحات -
تاریخ انتشار 1991